Effects of Extraction Buffer on the Solubility and Immunoreactivity of the Pacific Oyster Allergens

Despite recent technological advances, novel allergenic protein discovery is limited by their low abundance, often due to specific physical characteristics restricting recovery during the extraction process from various allergen sources. In this study, eight different buffers were compared for ability recover proteins Pacific oyster (Crassostrea gigas). The composition was investigated using high resolution mass spectrometry. antibody IgE-reactivity of each extract determined a pool serum five shellfish-allergic patients. Most showed good capacity oyster. general, higher concentration recovered salt or pH buffers, subsequently revealing more IgE-reactive bands on immunoblotting. contrast, resulted in poor and reduced IgE-reactivity. Discovery additional associated with an increase abundance extracts. conclusion, increasing ionic strength buffer improves solubility tissue. This strategy could also be applied other difficult-to-extract sources, thereby yielding improved panel increased diagnostic efficiency.